For analysis of small or very simple experiments, spreadsheet programs like Microsoft Excel are sufficient, and useful open-source tools exist for analysis and exploration of data from high throughput screens in general.
CELLPROFILER MEASURE FIBER LENGTH SOFTWARE
The volume and richness of individual-cell data from large image-based screens is unprecedented and existing software is inadequate for the challenge of data analysis. This tool has been useful for extracting image-based measurements to score sophisticated screens, with many more in progress.
We recently developed open-source image analysis software, CellProfiler, which measures a rich set of cellular features in images, such as size, shape, and staining patterns including intensity, texture, and colocalization. This capability is increasingly important given the ease now to systematically perturb cells with libraries of chemicals or gene-perturbing reagents like RNA interference or gene overexpression and collect hundreds of thousands of images of these cell samples. The scientific community has only begun to scratch the surface of computationally extracting the rich information visible in fluorescence microscopy images of cell samples.
Visual analysis of cell samples has played a dominant role in the history of biology.
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